rnovak at pharma.hr
Sat Jan 26 12:22:57 EST 2008
does anyone have experience with permeabilizing THP1-derived
macrophages (stimulated by PMA)? the 2% PFA and 0,25% saponine
combination works well on THP1 (monocyte like) cells, but this seems
to be too agressive for macrophages...i.e. all the cells fell apart up
to the analysis! anyone have any tips or suggestins?
Rudjer Novak, B.Sc.
University of Zagreb
Faculty of Pharmacy and Biochemistry
A. Kovacica 1, Zagreb
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