permeabilizing macrophages

Rudjer Novak rnovak at
Sat Jan 26 12:22:57 EST 2008

dear flowers,

does anyone have experience with permeabilizing THP1-derived 
macrophages (stimulated by PMA)? the 2% PFA and 0,25% saponine 
combination works well on THP1 (monocyte like) cells, but this seems 
to be too agressive for macrophages...i.e. all the cells fell apart up 
to the analysis! anyone have any tips or suggestins?


Rudjer Novak, B.Sc.
University of Zagreb
Faculty of Pharmacy and Biochemistry
A. Kovacica 1, Zagreb

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