Fwd: Appropriate number of events to collect for 3 color flow cytometry ?

Lora Barsky lbarsky at chla.usc.edu
Fri Jan 25 13:50:10 EST 2008


I find this chart is helpful to have around, when this question comes  
up.

Begin forwarded message:

> From: "Howard Shapiro" <hms at shapirolab.com>
> Date: January 20, 2008 10:43:31 AM PST
> To: "Cytometry Mailing List" <cytometry at flowcyt.cyto.purdue.edu>
> Subject: Re: Appropriate number of events to collect for 3 color  
> flow cytometry ?
>
>
>
> Maciej Simm wrote:
>> Abdul, this question (or it's variation) comes up frequently on the
>> list. I recommend checking out this archived post -
>> http://www.cyto.purdue.edu/hmarchiv/2001/0261.htm
>>
>> Maciej Simm
>>
>> On Jan 16, 2008, at 3:52 PM, Abdul Khan wrote:
>>
>>> Hello Again!
>>>
>>> I am sorry for posting too many emails in the forum, can't get any
>>> better advice than here.
>>>
>>> I am just wondering and interested to know that if there is any
>>> particular number of events (say 10,000 or 20,000) I need to collect
>>> while doing 3 color flow cytometry in whole blood.
>>>
>
> I'm glad Maciej dug up my old post; I was about to rewrite the same
> thing and post it again. The short answer is that the number of cells
> you need to collect depends on what you're looking for - how many  
> do you
> expect to find per unit volume of sample, or per fixed number of  
> WBC or
> nucleated cells - and on the level of precision you want, which  
> will be
> determined, as I and Mario pointed out, by the number of cells of
> interest you actually do count, according to Poisson statistics.
>
> -Howard
>
>
> -Howard
>

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