Qdot705 excitation at 488nm

WEHICytometry facs_copy at wehi.EDU.AU
Thu Jan 24 22:35:09 EST 2008


Pal,

I'm attaching a graphic that might give you a visual impression of  
the *relative* excitations.  Don't take too much notice of the  
absolute signal-to-background in each case because the data were  
acquired on a MoFlo with (at that time) not very red-sensitive  
detectors.  The staining was biotinilated B220 (CD 45R) and  
streptavidin quantum dots on mouse lymph node cells. The laser powers  
used were: 351-364 nm 20mW, 405 nm 35mW, 488 nm 300mW, 633nm 35mW,  
all measured after the MoFlo's beam steering towers.  I believe the  
QDot excitation should be linear in laser power.

The 720/40 filter should give you about 85% of what you'd get through  
a 700/40 band pass filter.

Frank Battye.

On 24/01/2008, at 7:05 PM, Pál Jáksó wrote:

> I'd like to know how efficient is the regular 15-20 mW 488 nm  
> excitation for Qdot705 nanocrystal compared to the optimal 405 nm  
> excitation?  I'd like to use it on a Partec CyFlow space with 20 mW  
> 488 nm laser and 720/40 bandpass filter.
>

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