Quantifying genome DNA

Gerstein, Rachel Rachel.Gerstein at umassmed.edu
Mon Jan 21 12:58:41 EST 2008


hi

do you mean 1N vs 2N genomes per cell (and <1 and >2) ? 

If so, this is a standard cell cycle analysis and cells fixed, permeablized and then treated with PI is the "gold standard" and I am sure you can easily find protocols.  There are a number of alternative fluors as well, including for live cell analysis.

=======================================================
Rachel M. Gerstein, Ph.D.
Associate Professor
Department of Molecular Genetics and Microbiology
Graduate Program in Immunology/Virology
University of Massachusetts Medical School
55 Lake Avenue North
Worcester, MA 01655-0002
(508) 856-1044
(508) 856-5920 (FAX) 



-----Original Message-----
From: Sripriya Subramaniam [mailto:sripriya at vasci.umass.edu]
Sent: Thu 1/17/2008 4:42 PM
To: cyto-inbox
Subject: Quantifying genome DNA
 
Hello everybody,

I have a student who is looking to  quantify entire genomic DNA by flow cytometry. Has anyone worked on this in the past ?

Any suggestions will be greatly appreciated.

- 

Sripriya Subramaniam
Flow cytometry facility
University of Massachusetts



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