Preventing Membrane Leakiness of Fixed Cells

JOHN BOUCHER jbouch at
Sat Jan 5 16:20:59 EST 2008

Dear LIST:

Earlier, I requested help for a method(s) to prevent membrane  
leakiness to fluorescent labels in fixed cells. Help may be on the  
way!  The goal is to label ONLY surface molecules of fixed cells  
WITHOUT the label penetrating the membrane and labeling inner lipid  
bilayer molecules also.

  A source potentially wanting to tackle the problem will work on a  
solution IF the need is great enough.  IF you are one who also needs  
this help, please let me know, AND include  any specialized needs  
(spectral requirements, or other parameters needed to measure at the  
same time).

Regards,	       John

John H. Boucher, DVM, PhD
Rheotech Labs, Inc.
Comparative Studies in Blood Rheology
2106 Salisbury Road
Silver Spring, MD 20910

Email:	<jbouch at>
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