CFSE staining

alireza badiei alirezadvm at hotmail.com
Sat Jan 5 05:06:59 EST 2008


Dear Seniors and Professors,
I am doing my MSc. in immunology, evaluation of microglial cells prolifration is part of
my project but i dont know if i should stain my cells with CFSE after harvesting of cells
as i am doing primary cell culture (rat) or after first pasage? What about the
concenration of CFSE? Any advise and protocol will be highly appreciated.
yours 
Alireza
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