Preventing Membrane Leakiness -- How To.
jbouch at verizon.net
Thu Jan 3 22:34:10 EST 2008
Michael took the words right out of my mouth (see below). I ,too,
need to find a technique for overcoming the membrane leakiness caused
by fixing RBCs (glutaraldehyde or formaldehyde). As with Michael,
improving the fixation technique to prevent/inhibit membrane pores,
OR, "building" a fluorescent label larger than the pores so that they
won't penetrate the membrane is a long sought goal.
Please, please, if you send a miracle answer to Michael, send a copy
to me too.
John H. Boucher, DVM, PhD
Rheotech Labs, Inc.
Comparative Studies in Blood Rheology
2106 Salisbury Road
Silver Spring, MD 20910, U.S.A.
Email: <jbouch at verizon.net>
From: Michael Model <mmodel at kent.edu>
Subject: plugging holes in cells
Date: January 2, 2008 1:59:12 PM EST
I am trying to do something to prevent penetration of a small (mw
under 1,000) hydrophilic molecule into chemically fixed cells. The
cells can be fixed with formaldehyde or glutaraldehyde for example,
but that makes their membranes leaky, so I am looking for a way to
either fix them without opening large holes or to somehow plug the
holes afterwards. Does anyone have any experience that might suggest
a possible approach?
Thanks in advance and happy New Year.
Michael Model, Ph.D.
Confocal Microscopy Core
Dpt. Biological Sciences
Kent State University
Kent, OH 44242
-------------- next part --------------
HTML attachment scrubbed and removed
More information about the Cytometry