plugging holes in cells

MODEL, MICHAEL mmodel at kent.edu
Wed Jan 2 13:59:12 EST 2008


Dear List:

 

I am trying to do something to prevent penetration of a small (mw under
1,000) hydrophilic molecule into chemically fixed cells. The cells can
be fixed with formaldehyde or glutaraldehyde for example, but that makes
their membranes leaky, so I am looking for a way to either fix them
without opening large holes or to somehow plug the holes afterwards.
Does anyone have any experience that might suggest a possible approach?

 

Thanks in advance and happy New Year.

 

Michael Model, Ph.D.

Confocal Microscopy Core

Dpt. Biological Sciences

Kent State University

Kent, OH 44242

tel. 330-672-2874

 

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