plugging holes in cells
mmodel at kent.edu
Wed Jan 2 13:59:12 EST 2008
I am trying to do something to prevent penetration of a small (mw under
1,000) hydrophilic molecule into chemically fixed cells. The cells can
be fixed with formaldehyde or glutaraldehyde for example, but that makes
their membranes leaky, so I am looking for a way to either fix them
without opening large holes or to somehow plug the holes afterwards.
Does anyone have any experience that might suggest a possible approach?
Thanks in advance and happy New Year.
Michael Model, Ph.D.
Confocal Microscopy Core
Dpt. Biological Sciences
Kent State University
Kent, OH 44242
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