ADCC flow based assay

Troche, Holly HTroche at dynavax.com
Wed Feb 13 17:27:51 EST 2008


Has anyone tried a flow based ADCC assay and what were your experiences
with developing this assay? I am attempting to do such an assay using
PKH-26 membrane dye to label the target cells, TOPRO-3 iodide to measure
viability during the assay, and annexin-v FITC to measure the amount of
apoptosis in  the target cells.

 

Holly Troche

Research Associate II

Preclinical Research

Dynavax Technologies Corp.

2929 Seventh St. Suite 100

Berkeley, CA 94710

htroche at dynavax.com| 510-665-0405

 

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