[Cytometry] Automated cell counters for sorting cores

Charles A Kuszynski ckuszyns at unmc.edu
Mon Dec 22 11:33:42 EST 2008


We are currently looking at a Nexelon unit.  The demo went pretty smoothly
and there is a stored image of the cells so you have "proof" that the cell
count was accurate or not and perhaps more importantly, the viability at
the start of the sort. Further, there are templates for the cells you use
most often and can create your own so that the counts are specific for
sizes of the cells rather than the debris.  There is no reason however that
our current method, hemocytometer and trypan blue, is not adequate for most
labs.

Charles A. Kuszynski, Ph.D.
Associate Professor
Director, Cell Analysis Facility
University of Nebraska Medical Center
985816 Nebraska Medical Center
Omaha, NE 68198-5816
402 559-6299 office
402 559-6267 lab
402 980-7654 cell
402 559-4069 fax
ckuszyns at unmc.edu

"What a long strange trip it's been" Grateful Dead

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             "Monica DeLay"                                                
             <Monica.DeLay at cch                                             
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                                                                   Subject 
                                       Automated cell counters for sorting 
                                       cores                               
                                                                           
                                                                           
                                                                           
                                                                           
                                                                           
                                                                           




This question is for all of the people who run cell sorting cores out
there.

We have discrepencies in cell counts from what people say they bring and
the threshold
count that we get and also in what the machine says it sorted and what they
count.  We
were thinking about getting an automated cell counter.             Is this
necessary or is the old
fashioned hemocytometer that we use ok?  Are any of the automated cell
counters any good
and if so which do people recommend?

Thanks!

Monica DeLay
Cincinnati Children's Hospital Medical Center
Department of Rheumatology
TCHRF 5543
3333 Burnet Ave
Cincinnati, Ohio 45229
Phone (513) 636-3575
Fax (513) 636-3328






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