GFP and Sapphire

Gerstein, Rachel Rachel.Gerstein at
Fri Sep 28 07:23:51 EDT 2007


check, there is a recent review by R. Tsien in Nature Methods.	I would guess Sapphire is like Vex, which we have used.  Vex is excited using a 407 nm laser.	We use compensation to correct for spillover caused by the small amount of excitation by the 488 nm laser.

Rachel M. Gerstein, Ph.D.
Associate Professor
Department of Molecular Genetics and Microbiology
Graduate Program in Immunology/Virology
University of Massachusetts Medical School
55 Lake Avenue North
Worcester, MA 01655-0002
(508) 856-1044
(508) 856-5920 (FAX) 

-----Original Message-----
From: Lindsey Laycock [mailto:llaycock at]
Sent: Wed 9/26/2007 8:29 PM
To: cyto-inbox
Subject: GFP and Sapphire


I am wondering where I can find the excitation/emission spectra of the
fluorescent protein T-Sapphire - does it exist?  We are finding that the
488 laser is exciting T-sapphire as much as or more so than the UV line
and thus hinders our ability to use GFP at the same time.  Would using a
lower laser power on 488 help with this?   


~Lindsey Laycock

BC Cancer Research Centre

Flow Cytometry Core Facility

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