Optic set up for multicolr assay on Mo-Flo (2 lasers -6 colors)
Bee Ling Ng
bln at sanger.ac.uk
Tue Sep 25 11:49:09 EDT 2007
I would be glad if you could share with me your 'optimal' optic
configuration (please include the type of Bandpass, longpass, short pass
as well as dichroic mirrors you are using) for the following combination
of fluorochromes on MO-FLO.
First laser 488nm(1st pinhole) : FITC, PE, PECY5.5, PECY7
Second Laser 635nm (3rd pinhole): APC, APC-CY7
Comment on the signals obtained is appreciated.
The wellcome Trust Sanger Institute
The Wellcome Trust Sanger Institute is operated by Genome Research
Limited, a charity registered in England with number 1021457 and a
company registered in England with number 2742969, whose registered
office is 215 Euston Road, London, NW1 2BE.
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