Fixed cells - washing out fixative before running?
a.smith at centenary.usyd.edu.AU
Mon Sep 24 22:25:06 EDT 2007
We've been having some problems with repeated (partial)clogging on
our LSR-II and are working through possible causes and solutions.
One thing that has emerged in the past couple of days is that there
is one group that runs fixed samples that are still sitting in the
fixative (10% neutral buffered formalin), ie they don't wash the
cells after fixing. There has been some suggestion that this might be
contributing to the problem. Has anyone ever run into problems with
people doing this? I've always washed cells after fixation and
resuspended them in something else (like PBS) so I've not considered
it before - it doesn't sound like it should create problems but I'm
interested to hear from others.
Incidently, the same group is having a lot of problems running the
same samples on an AMNIS Imagestream (the flow rate is unstable after
the first couple of minutes) - could the presence of formalin also be
an issue there?
Centenary Institute, Sydney, Australia
-------------- next part --------------
HTML attachment scrubbed and removed
More information about the Cytometry