Summary of results: 12um pore size 12well transwell plates

Alphen, Floris van f.vanalphen at sanquin.nl
Mon Sep 24 04:44:24 EDT 2007


Dear Flowers,

Thanks for all the replies to my question of last week concerning 12um pore size transwell plates. 

I've put all replies in this email for other peoples benefit.

We are going to test Millipore standing inserts to see if they can be used for our experiments

 

Floris

Floris van Alphen
Sanquin CLB
Dept. Molecular Cell Biology / Flow Cytometry
Plesmanlaan 125, 1066 CX Amsterdam
The Netherlands
+31(0)20-5123347
F.vanalphen at sanquin.nl


Hi Floris,
The BD 8um pore size plates ( 24 well inserts) are a good alternative as they still allow cells to go through if you are looking at the pore size for a metastasis assay. 

best regards Parul
Parul.Sharma at childrens.harvard.edu


Hi,
Check Nunc.
Dan 
dfreeck at guavatechnologies.com


Check with Millipore has all sorts of transwell inserts including 12um. http://www.millipore.com/catalogue.nsf/docs/C10504
I hope this helps...
Ali Borazjani
Biomedical Engineering
Dept. Ag & Bio Engineering
Life Sciences and Biotechnology Inst.
Mississippi State, MS 39762
Ph (662)312-7601


Hallo Floris: We use Neuroprobes chambers (12 wells) with polycarbonate or
cellulose nitrate filters.
T. Parra Cid
Unidad de Investigación
Hospital Universitario de Guadalajara


Hi Floris,

Greiner en BD (Falcon) hebben vergelijkbare platen, evenals Millipore. Falcon had recent enkele *maanden* geen platen beschikbaar, wat ons in vergelijkbare problemen bracht.

Je student(e) moet er erg op letten dat deze producten vergelijkbaar maar niet identiek zijn. Zelfs al mocht je dezelfde fitlermaterialen en poriegrootte vinden (onwaarschijnlijk), dan nog zullen de performance karakteristieken anders zijn.

Guy
Guy.Hermans at ablynx.com


Floris,
Dit vind ik met google:

Tissue Culture Inserts (12 Well) - Biocompare Buyer's Guide CORNING TRANSWELL MEMBRANE, PC, 12MM DIAM, 12UM PORE, TC-TREATED, STERILE Sigma-Aldrich, Material: polystyrene polycarbonate membrane Pore Size: 12.0 μm ... www.biocompare.com/matrix/13868/Tissue-Culture-Inserts-(12-Well).html - 55k


Maar dit zul jezelf ook wel gevonden hebben.
Groeten
Arie van der Marel
vandermarel at distrilab.nl


Dear Dr. van Alphen,

I thank you for your request below. I inform you that we do not have any 12um pore size inserts. However, we do sell a 12-well insert with 8um pore size that we recommend only for very big cells (like the tumor cells). In general what matter is the nucleus size and not really the cell size. The nucleus is less flexible than the cytoplasm and constitutes the limiting factor for a cell to go through the pores. Can I ask you which kind of cells you are planning to use?

I hope this will help a little.

Best regards,

Nathalie

Nathalie Suarez Gonzalez, PhD
BD Biosciences Scientific Support Europe
Tel: (+31) 010/711.48.00
Fax: (+31) 010/711.48.01
e-mail: help.biosciences at europe.bd.com http://www.bdbiosciences.com/


Hi Floris,

   want to remember we used them from Falcon while ago, not sure about the pore size but you can check in their website.

Hope it helps,
Lola
Lola.Martinez at cancer.org.uk


Hello Floris, have a look at Greiner Bio one, perhaps they have asimilar product. 

Grit 

grit.schade at leister.com


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