Platelet fixation vincent.shankey at
Sun Sep 23 23:28:40 EDT 2007

I would like to echo the comments previously made by Ian Dommick regarding 
platelet fixation. I strongly agree that how you treat (or not) platelets 
depends on the question being asked. To say that platelets should never be 
fixed ignores much work by Alan Michelson, and others looking at levels of 
platelet activation in situ or following in vitro activation. To place a 
link suggesting that platelets should be first separated from whole blood 
fails to recognize a large body of evidence (Ken Ault, others) that in 
vitro manipulation of platelets, particularly preparation of platelet-rich 
plasma, can significantly increase background levels of platelet 
activation. These are tricky buggers to work with, and the experimental 
design should carefully and thoughtfully reflect the experimental question 
being asked.

T. Vincent Shankey, Ph.D.
Advanced Technology Center
Beckman Coulter, Inc.
vincent.shankey at

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