Fc Blocking - Mutz-3

Gerstein, Rachel Rachel.Gerstein at umassmed.edu
Wed Sep 19 08:38:57 EDT 2007


Does the line express FcR's ? If so, you could block with mAB against FcR.   



=======================================================
Rachel M. Gerstein, Ph.D.
Associate Professor
Department of Molecular Genetics and Microbiology
Graduate Program in Immunology/Virology
University of Massachusetts Medical School
55 Lake Avenue North
Worcester, MA 01655-0002
(508) 856-1044
(508) 856-5920 (FAX) 



-----Original Message-----
From: Cody Allison [mailto:Cody.Allison at med.monash.edu.au]
Sent: Mon 9/17/2007 5:05 PM
To: cyto-inbox
Subject: Fc Blocking - Mutz-3
 
Hi,

I am currently working with the DC-like precursor cell line, Mutz-3. I am having some
trouble with non-specific binding of my isotype control antibodies. I am using FITC
(IgG2a), PE (IgG1) and PECY5 (IgG1) - conjugated antibodies. 

I do block the cells with FCS, but was wondering whether anyone has encountered this
problem with, or has any specific blocking protocols when using Mutz-3. Any advice would
be greatly appreciated.

cheers
cody


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