single cell sorting

Liza Bronner lbronne at
Fri Sep 14 14:43:38 EDT 2007

Hello Flowers,

I am working on single cell sorting on a FACSAria. I was wondering if 
anyone has any advice or tips or a protocol for single cell sorting.

How do I get just one cell per well? How do I count the cells that get 
sorted into a 96 well plate to ensure that I get just one cell per well?

My clients are interested in single cell plate sorting for use with 
PCR. Is a 96 well plate my best option as I do have access to smaller 
Taraski plates?

I have sorted calibrite beads into a 96 well plate. I can tell the Aria 
to sort 5, 10 , 20 events per well, but that does not mean that I get 
this many beads per well. I have had difficulty counting how many beads 
I get per well.

Thank you in advance for your help!

Best Regards,
Liza Bronner

Liza Bronner
Research Specialist- Flow Cytometry Core
Emory Vaccine Center
954 Gatewood Road
Atlanta, GA 30329

lbronne at office: 404.727.8619
cell: 706.254.4654

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