single cell sorting
lbronne at emory.edu
Fri Sep 14 14:43:38 EDT 2007
I am working on single cell sorting on a FACSAria. I was wondering if
anyone has any advice or tips or a protocol for single cell sorting.
How do I get just one cell per well? How do I count the cells that get
sorted into a 96 well plate to ensure that I get just one cell per well?
My clients are interested in single cell plate sorting for use with
PCR. Is a 96 well plate my best option as I do have access to smaller
I have sorted calibrite beads into a 96 well plate. I can tell the Aria
to sort 5, 10 , 20 events per well, but that does not mean that I get
this many beads per well. I have had difficulty counting how many beads
I get per well.
Thank you in advance for your help!
Research Specialist- Flow Cytometry Core
Emory Vaccine Center
954 Gatewood Road
Atlanta, GA 30329
lbronne at emory.edu office: 404.727.8619
Online Scheduler: evc-flowsched.rmy.emory.edu
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