FL2H in PI cell cycle analysis

Verity Software House verity at vsh.com
Fri Sep 7 17:30:59 EDT 2007

For some reason I did not get Yobou's email, just Arjmand's reply.  Arjmand
is correct in that you should use a linear parameter for cell cycle analysis
and you should check your cytometer to be sure you are getting a linear
response over the full scale.  You should also use the FL2-area (integrated
signal), this insures that you are getting "total" signal from each event
not just the peak signal.  We have a complete set of DNA Cell Cycle Analysis
Rules and would be happy to send you a copy if you are interested.

If you have any other questions about cell cycle analysis I would be happy
to work with you directly.

Best regards

Don Herbert

Technical Support Manager 

Verity Software House

Tech at vsh.com

From: arjmand [mailto:arjmand at farabi.tums.ac.ir] 
Sent: Wednesday, September 05, 2007 11:46 PM
To: cyto-inbox
Subject: Re: FL2H in PI cell cycle analysis


Linear amplification should be applied for cell cycle analysis in order to
have a good? distinction of different stages. Log amplification actually
expands week signals and compresses strong ones. So when you apply log
amplification your data are still there but not visible. 

hope this was a little help.

Alireza Ardjmand, M.Sc 

Hematology, Oncology 
and stem cell research Cent. 
Shariati Hospital 
Kargar Ave. 14114 
 <http://www.horc-bmt.org/> http://www.horc-bmt.org 

Tel: 982184902626 
Fax: 982188004140 
Mobile: 989352335919 

alternative email: 
ardjmar72 at yahoo.com 

19th Asia Pacific Cancer Conference 
http://apcc-2007.org <http://apcc-2007.org/>  

On Wed, 5 Sep 2007 04:04:25 -0700 (PDT), yobou wrote 
> Dear all 
> for FL2H histograms, should FL2H be set to linear or logarithmic mode
during PI analysis of cell cycle??actually I tried both situations but
interpretation of the results was different upon cell cycle analysis by
ModFit. When FL2H was set to linear mode, there was a strong G2 arrest, but
when it was set to logaritmic mode there was no such G 2 arrest. why? 
> thanks 

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