FL2H in PI cell cycle analysis
arjmand at farabi.tums.ac.ir
Wed Sep 5 23:45:36 EDT 2007
Linear amplification should be applied for cell cycle analysis in order to have a good?
distinction of different stages. Log amplification actually expands week signals and
compresses strong ones. So when you apply log amplification your data are still there but
hope this was a little help.
Alireza Ardjmand, M.Sc
and stem cell research Cent.
Kargar Ave. 14114
ardjmar72 at yahoo.com
19th Asia Pacific Cancer Conference
On Wed, 5 Sep 2007 04:04:25 -0700 (PDT), yobou wrote
> Dear all
> for FL2H histograms, should FL2H be set to linear or logarithmic mode during PI
analysis of cell cycle??actually I tried both situations but interpretation of the
results was different upon cell cycle analysis by ModFit. When FL2H was set to linear
mode, there was a strong G2 arrest, but when it was set to logaritmic mode there was no
such G 2 arrest. why?
This message has been scanned for viruses and
dangerous content by MailScanner, and is
believed to be clean.
-------------- next part --------------
HTML attachment scrubbed and removed
More information about the Cytometry