Elimination of liquid waste

Ray Hicks rh208 at cam.ac.uk
Sat Sep 1 07:21:37 EDT 2007

I used to use hypochlorite (Virkon), which worked well in the analysers, but broke down under vacuum on my sorter to fill the room with chlorine (the powder itself triggered asthma in one of my users, so it was far from ideal) - I switched to a quaternary amine - based product (TriGene), which works well in both vacuum and passive waste systems (for me), I get no noticable foaming from it on my DiVa, though it does foam a little on the Canto II - I use it at approximately 1:100 of final tank volume.



  ----- Original Message ----- 
  From: Roger Andries 
  To: Cytometry Mailing List 
  Sent: Friday, August 31, 2007 11:16 AM
  Subject: Re: Elimination of liquid waste

  Hai Frank,

  In our Lab, the department of "Environmental Care" proposed us to use "RBS AM35" (we purchase it at 'Chemical Products' in Belgium) to clean and decontaminate our materials. However, in the waste tank of 
  our Vantage SE, I cannot use it due to excessive foaming during (the continuous) waste aspiration.
  It's a concentrated alkaline cleaning and pre-decontaminating liquid, composed of quaternair ammonium, non-ionic tensio-active elements, phosphates, poly-phosphates. According to the French AFNOstandards (NF T 72-151, -171, -190, -301, -201) it is said to have bacterial, mould and virus killing activitires (against Aids-, hepatitis- and tuberculosis-virus too) and is much less hazardous for the environment .


  On 28 Aug 2007, at 11:05, Stephen Taylor - Porton Down wrote:


  Roger Andries
  Laboratory of Molecular & Cellular Therapy - Department of Physiology and Immunology
  Medical School of the Vrije Universiteit Brussel
  Building E - Room E251 - Laarbeeklaan 103 - B1090 Brussel
  Tel: 02/4774573 - Fax: 02/4774568 - email: randries at vub.ac.be

    Hi Frank,

    In our CL2 labs we use 10% hypochlorite in the waste reservoir then dilute this to drain with copious amounts of water (with goggles and plastic gowns on in case of splashes), which I guess is the standard method.

    For our high containment CL3 work (including that which is both high containment and genetically modified) it’s a bit more long winded.

    Firstly the waste tanks are inside the containment isolator with our MoFlo. The waste is poured into a large disposable plastic jar and 10% hypochlorite added for a contact time of 4 hours. This waste is then solidified with polymer granules and the jar is sprayed with hypochlorite and double-bagged in autoclaveable bags. These bags are again surface decontaminated and the bagged-container placed in the transfer hatch. The waste is then sent for autoclaving and finally disposed of in the general lab waste bin (which then goes on for incineration).

    Hope this helps



    From: WEHICytometry [mailto:facs_copy at wehi.EDU.AU] 
    Sent: 22 August 2007 05:37
    To: Cytometry Mailing List
    Subject: Elimination of liquid waste

    Sorry.  There's nothing like the mention of elimination of waste to bring down the tone of a discussion.  However, even the most sophisticated of cytometers need to do it.

    The issue is that we have sorters and flow cytometers running biohazardous or genetically engineered samples and the waste needs to be safely and legally disposed of (our containment level is PC2).	So far we have used bleach to decontaminate and, as I see it, the best way is to add bleach to the waste tank while it is still connected to the cytometer (or, even better, to add the bleach before running the samples).  Then, to empty the waste, it can be tipped or suctioned out with no danger of the splashes or drips carrying a viable contaminant from the samples. 

    But is that the best practice or is there an alternative? Is bleaching the "greenest" way to decontaminate or is autoclaving an alternative? If so, how do you do it?  The whole tank can't be autoclaved because of the level sensors.  How would you disconnect or decant without drips or splashes?

    What do those of you at PC3 level of containment do?

    If there is a better way to handle waste than we are currently handling it, I'm guessing someone has invented it already.  That's why I ask..

    Frank Battye. 

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