CD45RA and CD45RO

Daniel Marmer Daniel.Marmer at cchmc.org
Thu Oct 4 15:50:33 EDT 2007


We are currently evaluating the surface expression of CD3+4+ and CD3+8+ naïve and memory
cells (CD45RA and CD45RO, respectively), in a number of patient populations as well as
normal adult and pediatric controls.   As expected, there are three populations
demonstrated: a transitional population connecting the RA and RO populations.  Anyone
have experience standardizing a gating strategy to quantitate these populations,
especially the transitional.  

Regards,


Daniel Marmer, M.S., MT(ASCP)
Manager, Core Flow Cytometry Facility
Cincinnati Children's Hospital Med Center
Division of Hematology/Oncology
3333 Burnet Ave, Bldg R, ML 7015
Cincinnati, Ohio 45229

email:	  daniel.marmer at cchmc.org
Office:  (513)636-8614
Fax:	  (513)636-3861






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