Autocompensation concern

Marty Bigos mbigos at gladstone.ucsf.edu
Thu Jun 28 16:04:09 EDT 2007


Mario Roederer correctly reminded me that an earlier paper of his  
(Cytometry, 1986) had the same algorithm description. I was aware of  
it, but forgot - you get old. your memory goes...

Marty



On Jun 27, 2007, at 1:57 PM, Marty Bigos wrote:

> Hi-
>
> The basic algorithm of matrix inversion to calculate compensation  
> has been known since the first published paper on compensation by  
> Loken, Parks, et al.
>
> However, specific implementations might vary, for example,, adding  
> noise to the baseline, etc.
>
> So I think it is always a good idea to check the results on your  
> compensation controls.
>
> Marty Bigos, Director
> Gladstone Flow Core
>
> mail:
> J. David Gladstone Institutes
> 1650 Owens Street
> San Frnacisco CA 94158
>
> Phones:
> (office) 415-648-8346
> (mobile) 415-845-8450
>
> fax:
> 415-355-0855
>
>
>
> On Jun 26, 2007, at 11:10 PM, Rossi Ralph wrote:
>
>> Dear all, does anyone have experience with autocomp algorithms ,  
>> specifically , do you consider
>>	   it suitable to trust implicitly the compensation that is  
>> done ?  and not to check the results.
>>
>> thanks
>>
>>
>>
>> Regards
>> Ralph Rossi
>> Flow Facility Manager
>> Peter MacCallum Cancer Centre
>> r.rossi at pmci.unimelb.edu.au
>> Ph 96561955
>>




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