Problem with RNA Preps from ARIA Sorts

Ck Poon cpoon at medsfgh.ucsf.edu
Mon Jun 25 11:55:54 EDT 2007


Hello All,
I have a user who is sorting fresh PBMCs (from whole blood after ficoll) and
fresh mouse splenocytes with at 70um nozzle at 70psi on an ARIA. He usually
runs at a speed between 6k to 10k threshold events/sec.
He is seeing a degradation of RNA preps from cells sorted on the ARIA
compared to preps from cells sorted on a DiVantage.
Here are some other pieces of information:
- There are no UV lasers on both instruments.
- Sorting at 25psi with a 70um nozzle on the DiVantage.
- He is sorting into the same medium on both instruments.
- Viability of the sorted cells with Trypan blue gave very good viability.

Please ask if you need more specific answers.
Thank you for any input anyone may have.
Thanks,
Ck

Ck Poon
Division of Experimental Medicine
University of California, San Francisco
UCSF Box 1234
San Francisco, CA 94143-1234
----------------------------------------
Tel. 1-415-206-8132
Fax. 1-415-206-8200
E-mail: cpoon at medsfgh.ucsf.edu
Update Log: http://www.xanga.com/DEMFlowCore





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