linear regression analysis for two fluorescence parameters

Thu Jun 21 14:07:51 EDT 2007

Long time reader, first time poster... and I'm actually posting for one of my
core users:
"I am trying to determine the degree of correlation between two fluorescence
parameters within a single gated population.  Samples were acquired on a LSRII,
and the compensated and gated populations were defined using FlowJo.  I need to
determine whether the degree of expression for two markers on cells within the
defined population are co-variant.  I'd especially like to get an R value.  Is
there a way to extract the compensated X and Y fluorescence values from the
population of interest so I can analyze them with statistics software?	Thanks a
lot for your help."  

Mike Waring
Massachusetts General Hospital
Charlestown, MA 02129

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