effect of viability dyes on single cell culture of human lymphocytes

James Crowe james.crowe at Vanderbilt.Edu
Wed Jun 20 15:45:15 EDT 2007

I am interested in whether anyone has or has seen data (ie not just  
opinion) on:

Whether addition of one or multiple viability dyes to human  
lymphocytes being sorted as single cells for later culture and	
proliferation affects the ability to proliferate those single cells.

Certainly, sorting more live cells and excluding dead cells is	
desirable, but also it is generally thought PI and some other dyes  
exert a toxic effect on prolonged exposure.

J. Crowe

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