Choosing filter configurations for Qdots in Flow

Pizzo,Eugene Pizzo at NSO1.UCHC.EDU
Tue Jun 19 10:50:55 EDT 2007


Hi Steve,

So if you are limited to a violet trigon on your LSRII does this mean you can only get
three Qdot fluor's or can you excite some with your blue laser and use the blue laser
octagon for some qdot emissions simultaneously? Has someone configured an optimal
arrangement given the standard three Pmt trigon for violet and an octagon for the blue
laser?

Gene Pizzo 

-----Original Message-----
From: Steve Perfetto [mailto:perfetto at mail.nih.gov] 
Sent: Thursday, June 14, 2007 9:46 PM
To: cyto-inbox
Subject: Re: Choosing filter configurations for Qdots in Flow

Joanna,

Actually, the best strategy for each Qdot detector is the use of a sharp
dichroic mirror with a wide band pass filter. After much trial and error the
attached filter configuration illustrates this point. You can also visit our
web site at; 
http://www3.niaid.nih.gov/labs/aboutlabs/VRC/flowCytometryCoreLaboratory/
for additional information. If your current system has a green laser, the
QD525 will be very dim because of the narrow 515/20 filter. I would
recommend using QD545 with the filters as listed in the attachment.

SP


Stephen P. Perfetto, MS.,MT. (ASCP)
Manager, Core Flow Cytometry Facility
Vaccine Research Center, NIH
Building 40
40 Convent Dr., Room 5507
Bethesda, MD 20892-3015

email: sperfetto at nih.gov
Phone: (301) 594-8659

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On 6/14/07 11:11 AM, "Roberts Joanna" <joanna.roberts at epfl.ch> wrote:

>  
> Dear Flow Cytometry Community,
>  
> I have a question about filter configurations for Qdots in flow cytometry=




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