Choosing filter configurations for Qdots in Flow

Roberts Joanna joanna.roberts at epfl.ch
Thu Jun 14 11:11:25 EDT 2007


  

Dear Flow Cytometry Community, 

 

I have a question about filter configurations for Qdots in flow
cytometry. 

 

I would like to order a new instrument with a violet laser to measure
the full range of qdot fluorochromes. From quick talks with BD, I have a
list of the filters they suggest. They represent slightly narrower
signal ranges in just about all cases than the filters used by the
fellows in the paper by Chattopadhyay et al in Nature Immunology last
year. 

 

For those of you working with these reagents in flow, could you offer
your opinion on this matter? Would you recommend collecting more signal
(and probably doing a bit more 'inter-qdot' compensation) or would you
suggest taking the narrower band pass, collecting less signal and
needing less compensation? 

 

Here is a list of the bandpass filters suggested from BD as well as
those used in the paper mentioned above. 

 

		     Chattopadhyay et al       Suggestion from BD

QDOT800 	780/60 (750LP)		800/30

QDOT705 	705/70 (670LP)		710/50

QDOT655 	660/40 (640LP)		660/20

QDOT605 	605/40 (595LP)		610/20

QDOT585 	585/42 (570LP)		585/42

QDOT565 	560/40 (557LP)		560/40

QDOT525 	515/20			     525/50

 

 

Any and all recommendations, ideas and advice are appreciated. 

 

Thanks a lot to the organisers and moderators who run this list and all
the people who take the time to dish out their 2 cents- it is great!

 

Best wishes, 

Joanna

 

Flow Cytometry Core Facility

EPFL, Swiss Federal Institute of Technology

SV-SG, Station 15

CH 1015, Lausanne

Switzerland

 

+41 21 69 39 547

  

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