Measurement of HLA Antibodies

Zand, Martin Martin_Zand at URMC.Rochester.edu
Thu Jun 14 08:41:18 EDT 2007


Sam,

OneLambda offers a flow based alloantibody detection system consisting
of beads coated with HLA peptides for MHC class I and II, including
single antigen beads.  These kits are expensive, as they are largely
used for clinical transplantation cross-matching.  See their web site:
www.onelambda.com

Depending on your application, it is also possible to detect
donor-specific alloantibody using a panel of CD40L stimulated B cells of
known MHC specificities.  See our work:
http://www.blackwell-synergy.com/doi/abs/10.1111/j.1600-6143.2003.00637.
x

Hope this helps

Martin Zand

_____________________________ 
Martin S. Zand, MD, PhD 
Associate Professor of Medicine, Microbiology and Immunology 
Medical Director, Kidney and Pancreas Transplant Programs 
Co-Director, Center for Biodefense Immune Modeling 
University of Rochester Medical Center 
601 Elmwood Ave - Box 675 
Rochester, NY 14642 
Vox:  585-275-4517 
Fax:  585-486-1043 
Web:  Zand Lab Web Site 




-----Original Message-----
From: Sam Coker [mailto:Sam_Coker at pall.com] 
Sent: Tuesday, June 12, 2007 1:35 PM
To: cyto-inbox
Subject: Measurement of HLA Antibodies

Dear All:

Does anyone has a good flow cytometric method for measuring HLA (class I
and II) antibodies?

Thanks


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