FACS data presentation - density/contour plot

Joanne Lannigan joannelannigan at virginia.edu
Wed Jun 13 19:32:20 EDT 2007


Hi Julie:
I would refer you to the following publication by Roederer et al which will
answer your questions:

Methods Cell Biol. 2004;75:241-56
Guidelines for the presentation of flow cytometric data.
Roederer M, Darzynkiewicz Z, Parks DR.


Joanne Lannigan, MS
Director, Flow Cytometry Core Facility
University of Virginia
Jordan Hall, Room 7067
P.O. Box 800734
Charlottesville, VA 22908-0734
Office: 434-924-0274
Lab: 434-243-2695
Fax: 434-982-1071
email: joannelannigan at virginia.edu

> -----Original Message-----
> From: Julie Bertout [mailto:julie.bertout at ibl.fr]
> Sent: Wednesday, June 13, 2007 5:44 AM
> To: Cytometry Mailing List
> Subject: FACS data presentation - density/contour plot
> 
> Hello,
> 
> I have questions about density or contour plot :
> 
> how can you know which parameters (resolution, method, percentage,
> smoothing, ...)
> - were used when you read publicated FACS data?
> - you need to use to represent your data?
> what is the most common way to represent data? (method, percentage)
> 
> Thanks a lot,
> 
> Julie Bertout
> Flow Cytometry Core,
> Institut Pasteur de Lille - FRANCE





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