Summary: Identifying skin sub populations by flow

Roberts Joanna joanna.roberts at epfl.ch
Tue Jun 12 07:14:15 EDT 2007


Dear Flowers, 

 

I just wanted to say thanks so much to those of you who were able to
offer some suggestions regarding the identification of skin sub
populations. Your contributions were very much appreciated and are
summarised here. 

 

Thanks again, 

Joanna

 

a. 

If you are working on human skin, then CD1a is a terrific marker for
Langerhans cells.  There are quite a few antibodies available.	For the
other cell types, I'm not aware of specific cell surface markers that
are used in flow cytometry, although this may just be my ignorance.
Several different anti-cytokeratin antibodies are used commonly in
immunohistology for labeling keratinocytes, and these could work by flow
if the cells are permeablized.  

 

b.

I am not familiar with this either, but Langerhans: CD1a.

 

c.

I started to look at this a little while ago.  These are papers that I
thought may be useful...

 

 

Immunofluorescent surface labelling, flow sorting and culturing of
putative epidermal stem cells derived from small skin punch biopsies. 

M. M. van Rossum et al	2767 (2002) 109-117 Journal of Immunological
Methods

 

Prolonged MHC class II expression and CIITA transcription in human
keratinocytes

A Takagi et al 347(2006) 388-393 Biochemical and Biophysical Research
Communications

 

Marker succession during the development of keratinocytes from cultured
human embryonic stem cells

H Green et al  100 (2003) no. 26 15625-15630 PNAS

 

Single-cell expression profiling of human epidermal stem and
transit-amplifying cells: Lrig1 is a regulator of stem cell quiescence

K Jensen et al 103 (August 2006) no 32 11958-11963 PNAS

 

Immortalized keratinocyte lines derived from human embryonic stem cells

S Iuchi et al 103 (2006) no. 6 1792-1797 PNAS

 

Novel Functional Multiparameter Flow Cytometric Assay to Characterize
Proliferation in Skin

J. M. Mommers et al 42 (2000) 43-49 Cytometry (Communications in
Clinical cytometry)

 

Enrichment for murine keratinocyte stem cells based on cell surface
phenotype

H Tani et al 97 (2000) no. 20 10960-10965 PNAS

 

The cell-surface marker MTS24 identifies a novel population of
follicular keratinocytes with characteristics of progenitor cells

J G W Nijhof et al 133 (2006) 3027 - 3037 Development

 

 

 

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