Immune function panel

Masa Sasagawa masas at
Mon Jun 11 12:28:49 EDT 2007

Hi Natalie, interesting attempt.

Are you testing peripheral blood, other body fluids, or tissue samples?
I assume you are talking about PBMC. But then what types of cancer
patients, what stage, gender, age? Do you realize how many samples you
may need to reach sufficient statistical power for such broad analyses?
Are you investigating cytokines, cell typing, or both? There are diurnal
as well as menstrual variations of immune cell count and typing. You may
want to narrow down your focus more. 


Masa Sasagawa, ND
Naturopathic Physician - Washington
Affiliate Assistant Professor - University of Washington
  School of Nursing, Dept of Biobehavioral Nursing and Health Systems
Postdoctoral Research Fellow - Bastyr University
14500 Juanita Dr NE, #472
Kenmore, WA 98028
Phone: 425-602-3178
Fax: 425-602-3079
masas at

-----Original Message-----
From: Natalie Lassen [mailto:Natalie.Lassen at] 
Sent: Friday, June 08, 2007 4:09 PM
To: cyto-inbox
Subject: Immune function panel

immune function. I`d like to develop a panel of cytokines to evaluate
immune function status in healthy versus cancer-affected patients using
a Dako Cyan ADP flow cytometer.

I know I could start with a basic protocol including:
Cytokines: CD4, CD8/CD3/CD19/CD56

Would you recommend other specific ones that can be included?
Would you recommend specific literature that I can read?
Thank you in advance for your time.

My best regards,


> > Natalie Lassen, Ph.D.
> > Research Scientist
> > Phoenix, AZ 85016
> > Phone: 602.778.1944
> > Fax: 602.667.5623
> > natalie.lassen at

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