Decrease in MFI using HTS on LSRII

Ck Poon cpoon at medsfgh.ucsf.edu
Mon Jun 11 16:11:47 EDT 2007


Hello Andrew,

I don¹t know if this will answer your question.

We have two LSRII-HTS systems here. Over the years we discovered that the
rate at which you run the plate affects the outcome of the data. We don¹t
see major changes between 0.5ul/sec to 2.0ul/sec, but above 2.0ul/sec, the
FSC vs SSC profile seem to shift to the left and data seem to spread itself
out as well (haven¹t check to see if it spread toward the dimmer direction)


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