APC fluorochrome

Nancy Hurtado-Ziola nhurtado at UCSD.Edu
Sat Jun 9 15:51:09 EDT 2007

Hi Betty-Anne,

I'd love to find out what this is, too.  I've not seen it to often, but 
I've seen it.


On Jun 8, 2007, at 8:02 AM, bmcbey at uoguelph.ca wrote:

> I was wondering if anyone else has seen this problem.  I am using a 
> FACSAria for analysis with  three fluorochromes.  I've been finding 
> with my unstained sample that I regularly get a sharp peak in the APC 
> channel which is unlike the broader peak I get for cells stained with 
> the APC conjugated antibody.	I can get rid of this peak by 
> re-running	
> the sample after a period of 5 minutes or even if I put the sample on 
> ice for a few minutes.	It's kind of disturbing.  Does anyone have a 
> suggestion why this occurs?  I always run the cells through a cell 
> sieve filter top tube bought from BD to prevent plugging of the 
> machine.  Does this process non-specifically excite a cytoplasmic 
> molecule which is read in the APC channel?  That's the only 
> explanation I can come up with.
> Thanks!
> Betty-Anne McBey

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