Decrease in MFI using HTS on LSRII
andrew.morschauser at roche.com
Tue Jun 5 11:27:33 EDT 2007
I am posting the following question for a colleague:
Here is my question for the Flow cytometry group.
I have been trying to develop a plate based assay to measure a
cell-surface antigen on CHO cells using the LSRII-HTS system.
I perform the staining in 12X75mm polypropylene tubes. At the end of
the staining procedure, I transfer to polystyrene tubes and the mean
fluorescence of intensity is determined on the LSRII-HTS system. I then
take that same sample, transfer to polypropylene or polystyrene U-bottom
96-well plates and re-analyze the samples using the HTS system.
The MFI of the samples analyzed in the tubes is > than the MFI of the
samples analyzed in the plates? It is the same sample.
Shouldn't the MFI's be relatively the same?
I would like this to be a plate-based assay because there are quite a
number of samples to be evaluated but I don't feel comfortable with the
Any hints or suggestions would be greatly appreciated.
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