Isotype control reference
g.nebe-von-caron at unipath.com
Mon Jun 4 06:54:12 EDT 2007
[ Moderator's note:
[ Thanks, Gerhard; I am concerned about copyright, but I missed that the attachments in
[ the referenced message were published papers.
[ The links on our website have been changed to refer readers to the following URLs.
I would have been more worried about copyright issues when attaching the
pdf's. However with a good society comes a good journal that allows you
to access these articles directly on the web, a fact that can not be
> -----Original Message-----
> From: Ian Titley [mailto:Ian.Titley at icr.ac.uk]
> Sent: 01 June 2007 12:19
> To: Cytometry Mailing List
> Subject: Re: Isotype control reference
> Dear All
> After sending attachments with the message below I realised
> there was a risk of these being removed so here are the
> references "long-hand"
> Keeney et al 1998 Cytometry 34:280-283 and O'Gorman and
> Thomas 1999 Cytometry 38:78-80.
> Hi Rosie
> The two attached papers (pdf's), although now a bit dated,
> raise some interesting issues.
> Best wishes
> Ian Titley PhD
> Section of Haemato-oncology
> Institute of Cancer Research
> 15 Cotswold Road
> Surrey SM2 5NG
> Tel +44 (0)20 8722 4255
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> >>> Rosemary Clarke <r.z.clarke at dundee.ac.uk> 29/05/2007 16:46:05 >>>
> Hello everyone in flow land,
> A PhD student in the lab has recently had her viva and was asked why
> she didn't use isotype controls in her flow cytometry. There are
> technical reasons specific to the assays that made these unnecessary
> and irrelevant that we can easily explain. However, I was wondering
> if anyone out there has a reference that we can cite that explains
> the pitfalls of relying on isotype controls? I always feel it gives
> more weight to an argument if you can prove you are not the only one
> with a way of doing things!
> Thanks in advance
> Dr Rosie Clarke
> Division of Cell Biology and Immunology
> University of Dundee
> Tel: (01382) 385780
> E-mail: r.z.clarke at dundee.ac.uk
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