Viability Staining

Randy T. Fischer fischer1 at mail.nih.gov
Fri Jul 13 15:40:57 EDT 2007


Hi Liza,

Check the InVitrogen web site-they have various live/dead kits for the
different lasers, and depending on whether your samples are run fresh (can
use DAPI for this) or fixed.

Randy T. Fischer, NIH/NIAMS
B Cell Biology Group
9000 Rockville Pike
Bldg 10, Room 6D50
Bethesda, MD 20892
(301) 594-3537 (voice)
(301) 402-2209 (fax)


> From: Liza Bronner <lbronne at emory.edu>
> Date: Thu, 12 Jul 2007 16:58:22 -0400
> To: Cytometry Mailing List <cytometry at flowcyt.cyto.purdue.edu>
> Subject: Viability Staining
> 
> Hello to all,
> I am new to flow cytometry and I am trying to find a viability
> stain/probe that can be used with most samples run on a BD FACSAria. I
> am looking for something that I can ask all/most of my clients to use
> with their samples. Currently, some of my clients use 7-AAD or PI. I
> would like to find a stain that is excitable by the violet laser as
> most of my clients do not use dyes that involve the violet laser. Does
> anyone have any ideas? Thanks!
> 
> Liza
> 
> -- 
> Liza Bronner
> Research Specialist- Flow Cytometry Core
> Emory Vaccine Center
> 954 Gatewood Road
> Atlanta, GA 30329
> 
> lbronne at emory.edu office: 404.727.8619
> cell: 706.254.4654
> 
> Online Scheduler: evc-flowsched.rmy.emory.edu
> 




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