Custom LSR-II - laser options?
mbigos at gladstone.ucsf.edu
Tue Jul 3 16:05:12 EDT 2007
The 532nm @150 mW works quite well with mCherry and mTomato; we have
not had the chance to use mPlum. If you choose this option, you will
need to either use a narrower than standard detection filter for
FITC, or else buy a 532 notch filter. We have done the latter, and it
works quite will.
BD will also configure your system with a 561 nm laser. The power on
these diodes (50 mW) is less than the green, but they match the
excitation of PE and its tandems, and the new fluorescent proteins
better. I have not seen any comparison data to be able to say one
works better than the other.
Marty Bigos, Director
Gladstone Flow Core
J. David Gladstone Institutes
1650 Owens Street
San Frnacisco CA 94158
On Jul 2, 2007, at 6:47 PM, Adrian Smith wrote:
> Dear all,
> We are looking at options for a new custom-LSR-II and I'm keen to
> hear feedback on peoples' experience with different lasers.
> I'm sold on the green laser option for PE and its tandems. However,
> we are also very interested in being able to efficiently excite red
> fluorescent proteins like mCherry and mPlum and I'm wondering what
> the best approach is to configure an instrument to cover both options?
> What options are there in terms of lasers and in regards to
> configuring them on the LSR-II optical bench (how many lasers can
> you accommodate? has anyone got a "switching" setup working where
> they choose between different lasers lines? etc)
> Adrian Smith
> Centenary Institute, Sydney, Australia
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