Can cells express EYFP can do the BrdU pulse?

Gerstein, Rachel Rachel.Gerstein at umassmed.edu
Wed Jan 31 04:28:59 EST 2007


BD/Pharmingen has anti-BrDU that is labelled with APC.	 

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Rachel M. Gerstein, Ph.D.
Associate Professor
Department of Molecular Genetics and Microbiology
Graduate Program in Immunology/Virology
University of Massachusetts Medical School
55 Lake Avenue North
Worcester, MA 01655-0002
(508) 856-1044
(508) 856-5920 (FAX) 



-----Original Message-----
From: Junji Lin [mailto:junjilin at gmail.com]
Sent: Mon 1/29/2007 12:07 PM
To: cyto-inbox
Subject: Can cells express EYFP can do the BrdU pulse?
 
hi, I recently sorted some cells expressed GFP and do the cell cycle
analysis with it. Since most anti-BrdU primary or seconday antibodies are
FITC conjugated.This approach seems not feasible.Does anyone have
experiences on labeling GFP cells with BrdU and do cell cycle analysis? Or
you know if there are any companies making suitable antibodies for this kind
of analysis?
Thanks!

Junji Lin
University of Utah

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