Can cells express EYFP can do the BrdU pulse?

Junji Lin junjilin at gmail.com
Mon Jan 29 12:07:02 EST 2007


hi, I recently sorted some cells expressed GFP and do the cell cycle
analysis with it. Since most anti-BrdU primary or seconday antibodies are
FITC conjugated.This approach seems not feasible.Does anyone have
experiences on labeling GFP cells with BrdU and do cell cycle analysis? Or
you know if there are any companies making suitable antibodies for this kind
of analysis?
Thanks!

Junji Lin
University of Utah
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