Degradation of PeCy5.5 signal

Charland, Colette Colette.Charland at med.uvm.edu
Wed Jan 10 15:43:04 EST 2007


Greetings!

	I am having a problem with PeCy5.5 conjugated antibodies when
sorting.  It seems that the fluorophore is degrading as I get a decrease
in the PeCy5_5 signal with a concurrent increase in PE.  I am sorting
into Dulbecco's PBS pH 7.4.  Is anyone else seeing this?

Colette Charland
Researcher/Analyst
University of Vermont
89 Beaumont Ave.
Flow Cytometry Center/Given C316
Burlington, VT 05405
1-802-656-8742

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