Degradation of PeCy5.5 signal
Colette.Charland at med.uvm.edu
Wed Jan 10 15:43:04 EST 2007
I am having a problem with PeCy5.5 conjugated antibodies when
sorting. It seems that the fluorophore is degrading as I get a decrease
in the PeCy5_5 signal with a concurrent increase in PE. I am sorting
into Dulbecco's PBS pH 7.4. Is anyone else seeing this?
University of Vermont
89 Beaumont Ave.
Flow Cytometry Center/Given C316
Burlington, VT 05405
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