Analysis of rat broncheoalveolar lavage fluid (BALF)

Lanham, David LanhamD at
Fri Jan 5 10:04:14 EST 2007

Firstly, a happy new year to all the Purdue forum readers and remember to go
with the flow in 2007.

I have been looking for articles on leukocyte phenotyping analysis of rat
BALF.  There is a lot of information relating to alveolar macrophage
analysis, but I am interested in using the cytometer in place of the
traditional cytospin & differential count.  Can anyone out there point me to
some information or offer advice from their own experience?  We run Coulter
EPICS XL single laser (488nm) systems with 4 colour options (FL3 = ECD).

I look forward to your replies.

With best wishes


David F. Lanham
Department of Experimental Biology


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