problem with 733MHz Mac equipped Calibur

Ernest Stapleton Ernest.Stapleton at
Wed Jan 3 15:10:59 EST 2007

Hi there Justin,
Our FacsCalibur acts the same way. We are running the same software profile as you do.
Luckily it seems to have no adverse effects on the lasers, since they get shut off and re-started without a cool down. We still have our original 488 laser after 4 years (and running 7 hrs a day). Our HeNe was replaced once.
Otherwise, that's just the way they act. They CPU rarely crashes, so it's not a big annoyance.
I don't understand what you mean when you say you re-boot after data acquisition. Do you have to re-start after aquisition?
quote: Once data is collected and the Mac is rebooted,
If you are constantly rebooting your Mac, you could have an OS software problem. 
Ernest Stapleton 
Division Manager 
Immunology and Genetics Laboratories 
Room 1524 Health Sciences Center 
St. John's, Newfoundland 
A1B 3V6 Canada 

-----Original Message-----
From: Fishbaugh, Justin [mailto:justin-fishbaugh at]
Sent: Wednesday, January 03, 2007 12:57 PM
To: cyto-inbox
Subject: problem with 733MHz Mac equipped Calibur


We have a FACS Calibur controlled by a 733MHz G4 Mac.  Once data is collected and the Mac is rebooted, the Mac will not reconnect with the instrument.  The only way to establish communication between the Calibur and Mac is to power them both down and bring them back up from a cold start.	I am trying to determine whether this is normal for Caliburs equipped with a 733MHz Mac.  I have conflicting information from BD and other labs on our campus that use Caliburs.  The Mac is running OS 9.2, Cellquest 3.3, and BD Inits 3.4.2.  

Thank you for any helpful information. 


Justin Fishbaugh

Technical Director

Flow Cytometry Facility

Holden Comprehensive Cancer Center

University of Iowa


Iowa City, IA  52242

(319) 335-8103 Tel

(319) 335-8049 Fax

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