Elimination of liquid waste

WEHICytometry facs_copy at wehi.EDU.AU
Wed Aug 22 00:36:33 EDT 2007

Sorry.	There's nothing like the mention of elimination of waste to  
bring down the tone of a discussion.  However, even the most  
sophisticated of cytometers need to do it.

The issue is that we have sorters and flow cytometers running  
biohazardous or genetically engineered samples and the waste needs to  
be safely and legally disposed of (our containment level is PC2).  So  
far we have used bleach to decontaminate and, as I see it, the best  
way is to add bleach to the waste tank while it is still connected to  
the cytometer (or, even better, to add the bleach before running the  
samples).  Then, to empty the waste, it can be tipped or suctioned  
out with no danger of the splashes or drips carrying a viable  
contaminant from the samples.

But is that the best practice or is there an alternative? Is  
bleaching the "greenest" way to decontaminate or is autoclaving an  
alternative? If so, how do you do it?  The whole tank can't be	
autoclaved because of the level sensors.  How would you disconnect or  
decant without drips or splashes?

What do those of you at PC3 level of containment do?

If there is a better way to handle waste than we are currently	
handling it, I'm guessing someone has invented it already.  That's  
why I ask..

Frank Battye.

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