question about analog compensation using beads

Mario Roederer roederer at drmr.com
Tue Aug 7 18:14:44 EDT 2007


No, changing voltages on detectors that are not included in the  
compensation will not affect compensation.  Of course, changing  
voltages on those that are involved will invalidate it.

mr

On Aug 6, 2007, at 7:15 PM, Dahui You wrote:

> Hi, all:
> I have a question about the compensation using flowjo after  
> acquisition using BD CompBeads.
> When I acquire data on the flow cytometer, I have to change my SSC  
> PMT settings (which has been optimized for my cells) on the beads  
> to get the profiles in range. So my questions is when PMT settings  
> changed, will it affect the compensation later in FlowJo and why it  
> is or not?
> Thanks a lot.
> I am still confused about the concept behind analog compensation.
>
>
> Dahui You
> Department of Biological Sciences
> Louisiana State University
> Baton Rouge, LA 70803
> Tel:225-578-5960
> Fax:225-578-2594
>
>
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