fixative use with annexin V/PI apoptosis
bartonbe at UMDNJ.EDU
Fri Jun 30 08:22:25 EDT 2006
Me too, with similar probes.
How about a fixative with AlexaFluor 488- annexin V or JC-1? Anyone
have luck? I heard somewhere that 0.5% paraformaldehyde might work,
can someone confirm/deny?
Beverly E. Barton, Ph.D.
Department of Surgery/Division of Urology
UMDNJ-NJMS MSB G519
185 S. Orange Avenue
Newark, New Jersey 07103
E-mail bartonbe at umdnj.edu
On Jun 29, 2006, at 7:04 AM, stan ress wrote:
> Hi all,
> We wish to measure apoptosis/necrosis in target cells that have been
> stained and then fixed with 1% PFA. Conventional annexin V-FITC/PI kit
> does not work because of signal quenching. Has anyone had success with
> TACS annexin V-Biotin kit (R&D)? It can apparantly be used for this
> I would appreciate any feedback.
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