fixative use with annexin V/PI apoptosis

Beverly Barton bartonbe at UMDNJ.EDU
Fri Jun 30 08:22:25 EDT 2006


Hi,
Me too, with similar probes.
How about a fixative with AlexaFluor 488- annexin V or JC-1?  Anyone 
have luck?  I heard somewhere that 0.5% paraformaldehyde might work, 
can someone confirm/deny?
Many thanks.

Beverly

Beverly E. Barton, Ph.D.
Assistant Professor
Department of Surgery/Division of Urology
UMDNJ-NJMS  MSB G519
185 S. Orange Avenue
Newark, New Jersey 07103

Telephone			973-972-0662
E-mail				bartonbe at umdnj.edu
Telefacsimile		973-972-3892


On Jun 29, 2006, at 7:04 AM, stan ress wrote:

> Hi all,
>
> We wish to measure apoptosis/necrosis in target cells that have been
> stained and then fixed with 1% PFA. Conventional annexin V-FITC/PI  kit
> does not work because of signal quenching. Has anyone had success with
> TACS annexin V-Biotin kit (R&D)? It can apparantly be used for this
> purpose.
>
> I would appreciate any feedback.
>
> Thanks,
>
> Stan
>




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