Rat bronchoalveolar lavage fluid analysis

Tomas Navratil TNavratil at inspirepharm.com
Tue Jun 13 08:30:54 EDT 2006

Hello all,

Here is a question from a newbie: 

We just purchased a new Dako MoFlo. We are still waiting for the training. One of the applications would be a rat and mice bronchoalveolar lavage fluid analysis for the basic subtypes of inflammatory cells (lymphocytes, monocytes, neutrophils, eosinophils and basophils). Would you mind suggesting how to implement this analysis? What are the important determinants for the sample? Is it the volume, amount of cells or the cell concentration? Thank you for any useful advice. 

Kind regards,


Tomas Navratil, Ph.D. 
Senior Research Scientist
Inspire Pharmaceuticals, Inc. 
Suite 560, 4222 Emperor Boulevard 
Durham NC 27703 U.S.A. 
email: tnavratil at inspirepharm.com
phone: 919.941.9777 
mobile: 919.260.5094 

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