Ab labelling APC PE-Cy7 or APC-Cy7

Guy Hermans Guy.Hermans at ablynx.com
Sun Jan 22 10:47:53 EST 2006


Hi Claude,
 
as far as I know there are no ready-made tandem dye kits available other
than the Prozyme kit  <http://www.prozyme.com/phycolink/index.html>
http://www.prozyme.com/phycolink/index.html with Oyster dye rather than the
more common Cy/Alexa acceptor dyes. They have PE and APC kits, so you may
want to try APC or PE and pair that with the PE/Oyster dye tandem for the
second channel.
 
I haven't tried the tandem dye kit, but have had good results with their PE,
APC and PerCP kits.
 
Mario Roederer's website also lists PE tandem dye labeling procedures; check
out www.drmr.com <http://www.drmr.com/> 
 
Best of luck,
 
Guy
 



	 Next generation therapeutic  <http://www.ablynx.com/> antibodies

	

Guy Hermans, PhD
Project Leader	Ablynx NV
Technologiepark 4
B-9052 Zwijnaarde
Belgium 	
guy.hermans at ablynx.com 	
tel: 
fax: 
mobile: 	+32 (0)9 261 06 57
+32 (0)9 261 06 27
+32 (0)486 788 551 	
	

 <https://www.plaxo.com/add_me?u=30065269879&v0=994426&k0=2009290972> Add me
to your address book...   <http://www.plaxo.com/signature> Want a signature
like this?	

-----Original Message-----
From: Stéphanie Beq [mailto:sbeq at pasteur.fr] 
Sent: Friday, January 20, 2006 4:33 PM
To: cyto-inbox
Subject: Ab labelling APC PE-Cy7 or APC-Cy7


Dear flowers, 


A question from a collegue for you:

I would like to label these two antibodies, anti-mouse Dtk (Tyro 3) obtained
from R&D ref N° MAB 759, and anti-mouse Axl also from R&D ref ° MAB 8541. I
ordered purified Abs, and would like to label them with either APC, or
PE-Cy7, or APC-Cy7. Do you know a working "kit" or proccedure, knowing that
anti-Dtk is limited in quantity (500 microgr) and anti-Axl is also limited
(100 microgr).

Thanks,
Claude Roth
-- 
Claude Roth
Laboratoire Immunité Cellulaire Antivirale
Departement Immunologie
Institut Pasteur
28 rue du Dr Roux
75724 Paris Cedex 15
France
Tel: 33-1-40 61 32 71
       33-1-45 68 88 64
Fax: 33-1-45 68 89 42
e-mail: clroth at pasteur.fr








--------------------------------------------------------------

Beq Stéphanie PhD

Unité des Virus Lents

Département de Virologie

Institut Pasteur

25 rue du Docteur roux 

75724 cedex 15 Paris

FRANCE

tel: +33 1 40 61 32 24

Fax +33 1 40 61 31 67

____________________________________




-----------------------------------------------------------------------
THIS E-MAIL MESSAGE IS INTENDED ONLY FOR THE USE OF THE INDIVIDUAL OR
ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN INFORMATION THAT IS
PRIVILEGED, CONFIDENTIAL AND EXEMPT FROM DISCLOSURE.
If the reader of this E-mail message is not the intended recipient, you
are hereby notified that any dissemination, distribution or copying of
this communication is strictly prohibited.  If you have received this
communication in error, please notify us immediately at
ablynx at ablynx.com. Thank you for your co-operation.

"NANOBODY" and "NANOCLONE" are registered trademarks of Ablynx N.V.
-----------------------------------------------------------------------
-------------- next part --------------
HTML attachment scrubbed and removed
-------------- next part --------------
A non-text attachment was scrubbed...
Name: not available
Type: application/octet-stream
Size: 1030 bytes
Desc: not available
Url : https://lists.purdue.edu/pipermail/cytometry/attachments/20060123/737753ec/attachment-0002.obj


More information about the Cytometry mailing list