cells with gold nanoparticles
Marcel.Desrosiers at iaf.inrs.ca
Thu Feb 2 12:29:54 EST 2006
The reference you are looking for, goes back to 1987 when Roger Festin and all demonstrated that it was possible to used colloidal gold 40nm particles to surface stain T cells in conjunction with PE and FITC without interference with the fluorescence staining. The colloidal gold staining was detected onto the side scatter channel of the 488nm laser. This is the reference:
Detection of triple antibody-binding lymphocytes in standard single laser flow cytometry using colloidal gold, fluorescein and phycoerythrin as labels.
Festin R <http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Search&itool=pubmed_Abstract&term=%22Festin+R%22%5BAuthor%5D> , Bjorklund B <http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Search&itool=pubmed_Abstract&term=%22Bjorklund+B%22%5BAuthor%5D> , Totterman TH <http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Search&itool=pubmed_Abstract&term=%22Totterman+TH%22%5BAuthor%5D> .
Or the PuBMed links:
Hope that's help
Cytometry and Digital Imaging Core Laboratory
INRS-Institut Armand Frappier
Université du Québec
De : janet dow [mailto:jldow at unity.ncsu.edu]
Envoyé : 31 janvier 2006 16:35
À : Cytometry Mailing List
Objet : cells with gold nanoparticles
Dear Fellow Flowers:
I have a client who has contacted me about sorting cells with gold
nanoparticles in them. I have a moflo with a 488nm, uv, and 635
lasers on it. Can I do this and if so, with which laser and at what
wavelength will it fluoresce?
Just as an after thought-is this a new way to look for gold-it would
be great to use my machine for something that might actually make me
a little money???
As always-thanks so much in advance for the advice and support.
Laboratory Research Specialist and Manager
Flow Cytometry and Cell Sorting Facility
North Carolina State College of Veterinary Medicine
4700 Hillsborough Street
Raleigh, NC 27606
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