Calcium flux on LSR II
Colette.Charland at med.uvm.edu
Fri Feb 3 09:25:46 EST 2006
Greetings from a very wet Vermont!
Is anyone routinely doing Calcium flux on the LSRII? I am
having a problem with setting a tight baseline when looking at ratio vs.
time. It appears that there are some cells that are already in flux,
when the cells first go through the flow cell. I am unsure if it has to
do with the handling of the cells by the investigator, or if this is
operator error. I have carefully followed the instructions provided by
BD but this is a reoccurring problem. Any guidance or comments are
University of Vermont
89 Beaumont Ave.
Flow Cytometry Center/Given C316
Burlington, VT 05405
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