platelet cloud

Craig.Turner@nbs.nhs.uk Craig.Turner at nbs.nhs.uk
Wed Aug 30 04:11:39 EDT 2006


Sounds very much like platelet activation to me. I would try filtering
buffers, but it is possible that whatever buffer X is will cause platelet
activation, but that is only speculation since we don't know what is in it.

Craig Turner


-----Original Message-----
From: Lavergne Sidonie [mailto:sidoniel at svm.vetmed.wisc.edu]
Sent: 28 August 2006 16:18
To: cyto-inbox
Subject: platelet cloud


Good morning everybody,

I have a (potentially naive) question...

I used flow cytometry on platelets.
On the SSC/FSC graph, my platelets + PBS formed a nice
round tight cloud in the lower left corner.
Whe I incubated the platelets with the buffer X, the cloud
became pointy towards the upper right corner.
It therefore seems like my buffer X triggered an increase
in size and granularity in a subpopulation of platelets.

Could it be something else than activation?

I am not working on platelet activation at all. So I did
not stain for it. I was just wondering whether these kind
of changes on the SSC/FSC graph alone could be considered
pathognomonic for platelet activation.

If you have any book chapter or articles about this matter,
feel free to refer me to them.

Thanks,
Sidonie.



*
*
Sidonie Lavergne, DVM
University of Wisconsin
School of Veterinary Medicine
2015 Linden Dr W
Madison, WI 53706
608-263-5520

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